The modern pharmaceutics actively screens an immense diversity of substances occurring in plants and other natural resources in the search for new effective medicinal agents. The Global Institute for Bioexploration (GIBEX) established by joint efforts of Rutgers University and the University of Illinois (United States) represents the organizational core of international scientific community whose activity is directed towards the search and development of new medicinal preparations from natural raw materials. The basis of GIBEX activity is the transfer of modern screening technologies to countries and geographical regions characterized by remarkable biodiversity. The GIBEX goals are to encourage the search for new natural biologically active substances, to maintain biodiversity, and to monitor the natural resources conservation.

The tobacco plants ( L.) carrying the gene controlled by (Aocs)AmasPmas, the hybrid promoter that includes regulatory elements of the agrobacterial octopine and mannopine synthase genes, as well as plants controlled by the same promoter and , maize alcohol dehydrogenase gene intron were obtained. The presence of the gene intron did not significantly change the level of expression of the gene in plants. The analysis of expression of hepatitis B virus surface antigen (HBs-antigen) in transformed plants expressing the under the control of different promoters was made. The level of HBs-antigen in plants carrying the gene controlled by (Aocs)AmasPmas, the hybrid agrobacterium-derived promoter, was the highest in roots and made up to 0.01% of total amount of soluble protein. The level of HBs-antigen in plants carrying the gene controlled by the dual 35S RNA cauliflower mosaic virus promoter was the same in all organs of the plant and made up to 0.06% of the total amount of soluble protein. Hairy root and callus cultures of plants carrying the gene and expressing the HBs-antigen were obtained.

Transgenic plants as an alternative of costly systems of recombinant immunogenic protein expression are the source for the production of cheap and highly efficient biotherapeuticals of new generation, including plant vaccines. In the present review, possibilities of plant system application for the production of recombinant proteins for veterinary use are considered, the history of the "edible vaccine" concept is briefly summarized, advantages and disadvantages of various plant systems for the expression of recombinant immunogenic proteins are discussed. The list of recombinant plant vaccines for veterinary use, which are at different stages of clinical trials, is presented.

Two-year-old seedlings of licorice plant ( Fisch) were exposed to three degrees of water deficit, namely weak (60-70%), moderate (40-50%), and strong (20-30%) relative water content in soil, whereas control plants were grown in soil with 80-90% water content. Moderate and strong water deficit decreased the net photosynthetic rate, stomatal conductance, and biomass production. Water use efficiency and the root-to-shoot ratio increased significantly in response to water deficit, indicating a high tolerance to drought. Weak water deficit did not decrease root biomass production, but significantly increased the production of glycyrrhizic acid (by 89%) and liquiritin (by 125%) in the roots. Therefore, a weak water deficit can increase the yield of root medical compounds without negative effect on root growth.

As a multifunctional signaling molecule, melatonin (ML) is widely considered to induce the defense mechanism and increase the accumulation of secondary metabolites under abiotic stresses. Here, the effects of different concentrations of ML (100 and 200 µM) on the biochemical and molecular responses of L. in hydroponic conditions under 200 mM NaCl treatment were evaluated. The results showed that NaCl treatment impaired photosynthetic function and reduced plant growth by decreasing photosynthetic pigments and gas exchange parameters. NaCl stress also induced oxidative stress and membrane lipid damage, disrupting Na/K homeostasis and increasing hydrogen peroxide levels. NaCl toxicity decreased nitrogen (N) assimilation activity in leaves by reducing the activity of enzymes associated with N metabolism. However, adding ML to NaCl-stressed plants improved gas exchange parameters and increased photosynthesis efficiency, resulting in improved plant growth. By enhancing the activity of antioxidant enzymes and reducing hydrogen peroxide levels, ML ameliorated NaCl-induced oxidative stress. By improving N metabolism and restoring Na/K homeostasis in NaCl-stressed plants, ML improved N uptake and plant adaptation to salinity. ML increased the expression of genes responsible for the biosynthesis of withanolides (, , , , , and ) and, as a result, increased the accumulation of withanolides A and withaferin A in leaves under NaCl stress. Overall, our results indicate the potential of ML to improve plant adaptation under NaCl stress through fundamental changes in plant metabolism.