Morphological and Molecular Analysis of with spp
(rodent pinworm), a common nematode in the colon of rodents, has rarely been reported in humans. The morphological identification of some pinworm species is difficult, especially in those cases where only fragments of worms are recovered. We aimed to identify isolates of spp. in rodents using morphological and molecular approaches, and to compare them with human
Preparation and Development of an Immunochromatographic Test for Early Detection of Canine Visceral Leishmaniasis: A Preliminary Study
Canine visceral leishmaniasis (CVL), caused by (), is a serious parasitic disease. Domestic dogs in endemic regions act as primary reservoirs for the parasite. Early diagnosis, control, and regular screening of dogs are essential for effective disease management. This study aims to develop a practical, low-cost immunochromatographic test (ICT) for detecting specific anti- antibodies in domestic dogs.
Cutaneous Leishmaniasis in Diyala Province from Eastern Part of Iraq during the Period of 2011 to 2021
Cutaneous leishmaniasis (CL) is a neglected tropical disease endemic in Iraq. However, epidemiological data from Diyala Province are limited. Diyala province is located in eastern of Iraq, on the border with Iran, and adjacent to Kermanshah Province, Iran. We investigated the prevalence and distribution of CL in Diyala Province from 2011-2021.
Infection in Thailand (2013-2024): A Comprehensive Epidemiological Analysis: A Narrative Review
This study provides a comprehensive epidemiological analysis of prevalence in Thailand from 2013 to 2024, revealing significant regional disparities and key demographic risk factors. We provided a consolidated analysis of prevalence in Thailand, encompassing data from 2013 to 2024 by reviewing findings from international databases (PubMed, Google Scholar), and local Thai publications. The studies reported a striking average prevalence of 11.39% in the Northern region, with alarmingly high rates reaching 46.55% in Sukhothai's Satchanalai District, where children aged 1-4 years accounted for 83.33% of cases. In contrast, the Southern region showed a much lower average prevalence of 1.82%, influenced by hygiene practices, parental education, and sibling presence. The Central region's average prevalence of 4.74% was associated with age, gender assigned at birth, and parental education. Housing conditions, including inadequate sanitation and overcrowding, consistently exacerbated transmission across all regions. These findings underscore the urgent need for targeted public health interventions, including routine screenings for young children, enhanced hygiene education, and improved sanitation infrastructure, to combat infections throughout Thailand effectively. Reporting this epidemiological evidence is crucial for informing and guiding effective public health policies and interventions, ultimately contributing to the reduction of burden and improved population health.
The Current Status of Medical Parasitology in Iran: Developing a Roadmap, Structural Mapping, and Proposed Scenarios for the Future of This Field
We aimed to review and analyze the status of Medical Parasitology course including current problems and challenges on human resources, infrastructure, research capacities, educational and investigative priorities in Iran, and to develop a roadmap and future development in this field.
Molecular Characterization of by Gene of India and Management of Its Infection in Cattle: A Case Report
A 3-year-old cow which suffered from anorexia and high fever for the last 2 days was investigated at farmer door step. Detailed examination of cow showed high temperature (40.5 °C, pale conjunctival mucous membrane, increased size of pre-scapular lymph nodes and severe tick infestation. Examination of blood smear showed the presence of spp. Haematological values showed low value of hemoglobin (4 gm/dl), TEC (2.3×10/μl), PCV (14%) with mild leukocytosis (14.5×10/μl). The biochemical values were almost within the normal reference range except for AST (88U/L). PCR amplification of the gene was done from genomic DNA which was isolated from cattle blood and the results showed amplification of approx. 270 bp. In silico analysis of the generated DNA sequence confirm the species of the parasite as and the phylogenetic inference of the generated sequence (MH178373) showed cladding with infecting domesticate bovines. This study proposed the use of heat-shock protein 70 () gene of species for inter-species characterization and phylogeny. Treatment with intra-muscular single dose of Buparvaquone (Intas Pharmaceutical Pvt. Ltd., India; 2.50mg kgalong with intra-muscular anti-pyrectic drugs Meloxicam and Paracetamol combination (Intas Pharmaceutical Pvt. Ltd., India;15 mg kg q24)for 2 days and oral preparation of haematinics (Intas Pharmaceutical Pvt. Ltd., India; 50 ml q24) for 10 days. This is the first report on molecular detection of infection from cattle in Tripura, as per available scientific literature.
Effectiveness of Attractive Toxic Sugar Baits (ATSB) in Reducing Cutaneous Leishmaniasis among Military Personnel in Isfahan Province, Central Part of Iran
We aimed to evaluate the effectiveness of Attractive Toxic Sugar Baits (ATSB) in reducing the incidence of Cutaneous leishmaniasis (CL) among military personnel in Isfahan Province, Iran.
Prevalence of Intestinal Microsporidia in Patients with Intellectual Disabilities in Rehabilitation Centers of Hormozgan Province, Southern Iran
The present study was carried out to investigate the prevalence of intestinal microsporidia in patients with intellectual disabilities in the rehabilitation centers of Hormozgan Province in south of Iran, using microscopic and molecular methods.
Genetic Identification of in Slaughtered Domestic Animals from Two Northeastern Iranian Cities Using HRM and Sequencing Techniques
Hydatidosis is one of the common parasitic diseases common to both humans and animals with global spread. The disease is very important from both public health and economic perspectives. The parasite presents with highly diverse genetic features, including a different genotype which affect the life cycle of the parasite, its transmission routes, pathogenicity, immunogenicity, response to drugs, epidemiology and disease control. Therefore, we aimed to identify the genotype of hydatid cysts isolated from slaughtered animals in Sabzevar and Neyshabour slaughterhouses by sequencing and high-resolution melting curve analysis.
Evaluation of Apoptotic Effect of Transgenic Expressing MLLO-Bax-Smac Fusion Gene in Infected Macrophages, an In Vitro Study
In the leishmaniasis parasitic infection, parasite escapes from the immune system of host or prevents macrophage apoptosis. It seems generating transgenic parasites to express proapoptotic proteins can accelerate the apoptosis of infected macrophage and prevent differentiation. Hence, we investigated the efficacy of transgenic expressing mLLO-BAX-SMAC in expediting macrophage apoptosis.
Synergistic Effects of Cold Atmospheric Multiple Plasma Jet and Amphotericin B on : An In-Vitro Study
This study aimed to assess the effects of Cold Atmospheric Multiple Plasma Jet (CAMPJ) on .
The Recent Human Case of in Iran: Could Be a Sign of Re-Emerging Scenario?
Beef tape worm a zoonotic helminth of platyhelminth phylum, is reported here in a 28-year-old female patient from Tehran, Iran taking advantage of microscopic and molecular techniques. The occurrence of this infection has been recorded high in previous decades in Iran while its record nowadays looks rare in the country. Two 18s and Cox1 fragments were amplified and sequenced successfully using specific primers. This case underscores the potential for transmission in the region, warranting rigorous slaughterhouse inspections and enhanced public awareness efforts.
Diffuse Cutaneous Leishmaniasis Caused by as the Initial Presentation of HIV Misdiagnosed as Scabies and Kaposi's Sarcoma: A Case Report
Diffuse cutaneous leishmaniasis (DCL) is a rare and severe form of cutaneous leishmaniasis (CL), particularly uncommon in Uzbekistan. Leishmaniasis remains a major opportunistic infection in immunocompromised individuals, especially in those with HIV, where co-infection with spp. can result in atypical clinical manifestations, delayed diagnosis, and poor treatment response. This is a retrospective descriptive case report of a single pediatric patient. We report the first known case of DCL in an HIV-infected pediatric patient in Uzbekistan. A 14-year-old boy presented with multiple, non-ulcerative, progressively worsening skin lesions on the face and extremities over six months. Although he had no travel history to known endemic areas, he resided in a region with reported local transmission. Physical examination revealed extensive infiltrative plaques and nodules. Laboratory investigations, including Giemsa staining and PCR, confirmed infection. Histopathological analysis showed both intravascular and extravascular amastigotes, and species identification revealed . Imaging ruled out visceral involvement. Given the patient's immunosuppressed status and the disseminated skin involvement, a diagnosis of DCL was established. Liposomal amphotericin B was initiated as first-line therapy. This case illustrates the diagnostic complexities of leishmaniasis in HIV-positive patients, where initial misdiagnoses (e.g., scabies or Kaposi's sarcoma) may delay appropriate treatment. It highlights the critical need for heightened clinical suspicion, better diagnostic tools, and improved physician awareness in endemic and emerging transmission areas. In the context of rising HIV rates and growing domestic tourism to endemic zones, early screening and structured public health interventions are essential to reduce disease burden and improve patient outcomes in Uzbekistan.
Molecular and Pathological Detection of in Aborted Fetuses of Sheep and Goats in East Azerbaijan Province, Northwest Iran
is recognized as one of the most important causes of abortion in small ruminants globally. This study was conducted to evaluate in aborted fetuses of sheep and goats in East Azerbaijan Province, northwest Iran.
Evaluation of Benzimidazole Resistance in Equine Cyathostomins in the Kermanshah Province of Iran Using Coprological Analysis and Allele-Specific PCR
Resistance to benzimidazole (BZ) by cyathostomin nematodes has become a major threat to equine health around the world. We aimed to evaluate the efficacy of BZ drugs against small strongyle nematodes in horses of western Iran using coprological and molecular examination.
Molecular Detection of Spp. Infection and Haemato-Biochemical Alterations Associated with It in Domestic Cats
Cytauxzoonosis is a tick borne hemoprotozoal disease of wild and domestic felids. Its epidemiology and clinical importance is less appreciated in India. The aim of this study was to diagnose the disease in domestic cats and identify the associated clinico-haemato-biochemical changes.
Seroepidemiology of Chronic Toxoplasmosis in Multiple Sclerosis Patients in Northeastern Iran
is a common intracellular parasite implicated in chronic infections that may contribute to the pathogenesis of multiple sclerosis (MS). The potential role of in triggering or exacerbating neuroinflammatory processes has been suggested in several recent studies. We aimed to assess the seroprevalence of anti IgG antibodies in MS patients compared to healthy individuals and to evaluate associated demographic, environmental, and lifestyle risk factors.
In Vitro Antimalarial Activity of Chloroquine-Crocus Sativus Conjugated to Chitosan Nanocomposits against 3D7 and K1 Strains of
The use of nanocarriers in combination with other treatments shows significant promise in addressing drug-resistant diseases, particularly malaria. Given the high prevalence of drug-resistant malaria, research into innovative therapies is crucial. This study focuses on a nanoform of chitosan, a biodegradable polymer, combined with (saffron) and chloroquine to enhance their antimalarial effects.
In Silico Characterization and Epitope Mapping of Annexin Protein: Novel Insights for Vaccine Design
A neglected zoonosis, cystic echinococcosis (CE) is the most common diseas in the developing nations worldwide. Vaccination is helpful in preventing the disease. Predicting main biochemical properties of the annexin (ANX) and its possible B-cell and human leukocyte antigen (HLA)-binding epitopes as a viable vaccine candidate was the goal of the current study.
Presence and Interactions of Lectin and Protein in Colorectal Cancer
We aimed to analyze the presence of lectin light chain antigen and protein in colorectal cancer biopsies to uncover potential antigenic interactions between them.
Prevalence of and spp. Co-Infection in Dust from Public Parks in Eastern Thailand
We investigated the prevalence and environmental factors associated with co-infection of and spp. in dust from public parks in Eastern Thailand, where industrial activity and air pollution may enhance pathogen survival and pose public health risks.
